Efek Pemberian Streptozotocin Dosis 150 mg/kgBB terhadap Perubahan Kadar Glukosa Darah dan Berat Badan pada Mencit Sebagai Model Diabetes Mellitus

Choirotussanijjah -; Renny Novi  Puspitasari; Rokhmatul  Asiyah

Authors

Choirotussanijjah - Universitas Nahdlatul Ulama Surabaya
Renny Novi Puspitasari Universitas Nahdlatul Ulama Surabaya
Rokhmatul Asiyah Universitas Negeri Malang

Abstract

Abstract : Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia, which continues to increase in prevalence worldwide and poses a major public health challenge. Streptozotocin STZ is commonly used as a diabetogenic agent in experimental studies due to its selective cytotoxicity toward pancreatic cells. This study aimed to evaluate the effects of STZ administration at a dose of 150 mg/kg body weight on random blood glucose levels and body weight in mice. An experimental design was conducted using positive and negative control groups. Mice were acclimatized for one week before STZ was administered intraperitoneally following an overnight fast. Blood glucose levels and body weight were measured weekly for 28 days. The results demonstrated a significant difference between the two groups. The positive control group exhibited marked hyperglycemia with a mean random blood glucose of 426.47 mg/dL, compared to 140.91 mg/dL in the negative control group. Moreover, body weight decreased in the positive control group (mean 25.1 g) compared to the negative control (34.26 g). Statistical analysis using the Mann-Whitney test confirmed significant differences (p greather than 0.05) in both parameters. In conclusion, administration of STZ at 150 mg/kg BW effectively induced hyperglycemia and weight reduction in mice, validating its reliability as an experimental model of diabetes mellitus.

Abstrak : Diabetes melitus merupakan gangguan metabolik kronis yang ditandai dengan hiperglikemia, dengan prevalensi yang terus meningkat secara global sehingga menjadi tantangan besar kesehatan masyarakat. Streptozotocin (STZ) banyak digunakan sebagai agen diabetogenik pada penelitian eksperimental karena efek sitotoksiknya yang selektif terhadap sel pankreas. Penelitian ini bertujuan mengevaluasi efek pemberian STZ dosis 150 mg/kgBB terhadap kadar glukosa darah acak dan berat badan pada mencit. Desain penelitian eksperimental digunakan dengan membagi hewan coba ke dalam kelompok kontrol positif (n = 8) dan negatif (n = 8). Mencit diaklimatisasi selama satu minggu sebelum diberikan STZ secara intraperitoneal setelah dipuasakan semalaman. Kadar glukosa darah dan berat badan diukur setiap minggu selama 28 hari. Hasil penelitian menunjukkan adanya perbedaan signifikan antara kedua kelompok. Kelompok kontrol positif mengalami hiperglikemia dengan rata rata kadar glukosa darah 426,47 mg/dL, dibandingkan 140,91 mg/dL pada kelompok kontrol negatif. Selain itu, berat badan mencit pada kelompok kontrol positif menurun (rata rata 25,1 g) dibandingkan kontrol negatif (34,26 g). Analisis statistik dengan uji Mann Whitney menunjukkan perbedaan bermakna (p lebih besar 0,05) pada kedua parameter. Simpulan penelitian ini adalah bahwa STZ dosis 150 mg/kgBB efektif menginduksi hiperglikemia dan penurunan berat badan pada mencit, sehingga valid digunakan sebagai model eksperimental diabetes melitus.

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