Comparative Evaluation of Clove Extract and Green-Synthesized Selenium Oxide Nanoparticles for Protection Against UV-B Induced DNA Damage: A Comet Assay-Based Study
DOI:
https://doi.org/10.17977/um067v6i22026p3Keywords:
Biosynthesis, Nano Selenium, Clove, Ultraviolet Array, Antigenotoxicity, Comet AssayAbstract
Ultraviolet (UV) radiation is one of the major environmental factors which can be harmful for cellular DNA. It can lead to genetic mutations and damage to DNA, which can increase the risk of a variety of diseases, including cancer. The objective of the present study was to evaluate the therapeutic effects of clove extract (Syzygium aromaticum), clove-mediated selenium nanoparticles (SeNPs) and clove extract alone on UVB-induced DNA damage in mice. UVB exposure resulted in a significant increase in DNA damage as measured by the Olive Tail Moment in the Comet assay. Post-treatment with clove extracts reduced DNA damage however, the clove mediated selenium nanoparticles showed a stronger therapeutic effect. The SeNP-treated groups, especially after 15 min exposure, showed the lowest Olive Tail Moment values, indicating a significant reduction in genotoxicity. This suggests that clove mediated selenium nanoparticles have great therapeutic potential in repairing UV induced DNA damage and improving genome stability.
References
Abid, N., Khan, A. M., Shujait, S., Chaudhary, K., Ikram, M., Imran, M., Haider, J., Khan, M., Khan, Q., & Maqbool, M. (2022). Synthesis of nanomaterials using various top-down and bottom-up approaches, influencing factors, advantages, and disadvantages: A review. Advances in Colloid and Interface Science, 300, 102597.
Akhtar, S., Najafzadeh, M., Isreb, M., Newton, L., Gopalan, R. C., & Anderson, D. (2020). Ex vivo/in vitro protective effect of myricetin bulk and nano-forms on PhIP-induced DNA damage in lymphocytes from healthy individuals and pre-cancerous MGUS patients. Archives of Toxicology, 94(7), 2349–2357.
Alfikri, F. N., Pujiarti, R., Wibisono, M. G., & Hardiyanto, E. B. (2020). Yield, quality, and antioxidant activity of clove (Syzygium aromaticum L.) bud oil at the different phenological stages in young and mature trees. Scientifica, 2020(1), 9701701.
Atanasov, A. G., Zotchev, S. B., Dirsch, V. M., & Supuran, C. T. (2021). Natural products in drug discovery: Advances and opportunities. Nature Reviews Drug Discovery, 20(3), 200–216. https://doi.org/10.1038/s41573-020-00114-z
Barboza, J. N., da Silva Maia Bezerra Filho, C., Silva, R. O., Medeiros, J. V., & de Sousa, D. P. (2018). An overview on the anti-inflammatory potential and antioxidant profile of eugenol. Oxidative Medicine and Cellular Longevity, 2018(1), 3957262.
Braafladt, S., Reipa, V., & Atha, D. H. (2016). The comet assay: Automated imaging methods for improved analysis and reproducibility. Scientific Reports, 6(1), 32162.
Cadet, J., & Douki, T. (2018). Formation of UV-induced DNA damage contributing to skin cancer development. Photochemical & Photobiological Sciences, 17(12), 1816–1841.
Cruz, L. Y., Wang, D., & Liu, J. (2019). Biosynthesis of selenium nanoparticles, characterization and X-ray induced radiotherapy for the treatment of lung cancer with interstitial lung disease. Journal of Photochemistry and Photobiology B: Biology, 191, 123–127.
Choudhary, M.K.; Kataria, J.; Cameotra, S.S. and Singh, J. (2015). A facile biomimetic preparation of highly stabilized silver nanoparticles derived from seed extract of Vigna radiate and evaluation of their antibacterial activity. Applied Nanosciences. 6 (1): 105-111
ElObeid, T., Yilmaz, M. T., Ispirli, H., Sagdic, O., Taylan, O., Basahel, A., Karaboga, D., Durak, M. Z., & Dertli, E. (2025). Biosynthesis of alternan-stabilized selenium nanoparticles: A study on characterization and applications for antibacterial and antifungal purposes. Inorganic and Nano-Metal Chemistry, 55(7), 793–806.
Ezeonu, C. S., & Ejikeme, C. M. (2016). Qualitative and quantitative determination of phytochemical contents of indigenous Nigerian softwoods. New Journal of Science, 2016(1), 5601327.
Garba, Z. N., Zhou, W., Lawan, I., Xiao, W., Zhang, M., Wang, L., Chen, L., & Yuan, Z. (2019). An overview of chlorophenols as contaminants and their removal from wastewater by adsorption: A review. Journal of Environmental Management, 241, 59–75.
Gudkov, S. V., Gao, M., Simakin, A. V., Baryshev, A. S., Pobedonostsev, R. V., Baimler, I. V., Rebezov, M. B., Sarimov, R. M., Astashev, M. E., & Dikovskaya, A. O. (2023). Laser ablation-generated crystalline selenium nanoparticles prevent damage of DNA and proteins induced by reactive oxygen species and protect mice against injuries caused by radiation-induced oxidative stress. Materials, 16, 5164.
Hwang, E. S., Lin, P. L., Ngo, H. T., & Yi, T. H. (n.d.). Clove attenuates UVB-induced photodamage and repairs skin barrier function in hairless mice.
Jackson, S. P., & Bartek, J. (2009). The DNA-damage response in human biology and disease. Nature, 461(7267), 1071–1078.
Lemenkova, P. (2019). Numerical data modelling and classification in marine geology by the SPSS statistics. International Journal of Engineering Technologies (IJET), 5(2), 90–99.
Pfeifer, G. P. (2020). Mechanisms of UV-induced mutations and skin cancer. Genome Instability & Disease, 1(3), 99–113.
Roy, S. (2017). Impact of UV radiation on genome stability and human health. In Ultraviolet Light in Human Health, Diseases and Environment (pp. 207–219).
Shirankar, S. S., Dongre, S. G., Limsay, R. P., & Somkuwar, A. P. (2018). The preliminary phytochemical screening of various leaf extracts of plant Limonia acidissima Linn. OPEN ACCESS POLICY, 47(3), 1321–1329.
Talaie, A., & Moussavi, S. M. (2025). The impact of ultraviolet radiation on human health. Journal of Environmental Treatment Techniques, 12(1), 39–66.
Zhang, Y., Liu, X., Wang, Y., & Chen, H. (2020). Extraction optimization of phenolic compounds from Syzygium aromaticum and evaluation of antioxidant activity. Journal of Applied Research on Medicinal and Aromatic Plants, 18, 100247. https://doi.org/10.1016/j.jarmap.2020.100247.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Kawther Y. Mashjal , Hussain S. Hasan , Labeeb Ahmed Alzubaidi
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1.png)
4.png)
