Fabrication and characterization of Chitosan Nanocarriers for Erucin Delivery Using Ionic Gelation Techniques
DOI:
https://doi.org/10.17977/um067v6i62026p3Keywords:
Chitosan Nanoparticles, Erucin, Ionic Gelation, Eruca Sativa , Drug Delivery SystemAbstract
Background: The incorporation of nanotechnology and bioactive molecules represents a boundary in modern pharmacology, aiming to improve the bioavailability and stability of plant-derived chemicals. This study focuses on the evolution of a novel drug delivery system using chitosan nanocarriers to encapsulate erucin, a potent isothiocyanate derived from Eruca sativa known for its significant anticancer, anti-inflammatory, and antimicrobial properties.
Methods: Fresh chopped Eruca sativa leaves were macerated in water to get erucin phytochemical by enzymatic conversion with myrosinase , followed by an organic solvent extraction using dichloromethane. In the production of nano erucin, sodium tripolyphosphate -a cross-linker - was used in a process called ionic gelation. Three definite solutions were prepared with chitosan-to-erucin ratios of 0.5:1, 1:1, and 1.5:1. Then, centrifugation performed to purify the resulting nanoparticles which were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), and Zeta potential analysis to evaluate morphology, elemental composition, and surface charge, respectively
References
Akdaşçı, E., et al. (2025). Chitosan and its nanoparticles: A multifaceted approach to antibacterial applications. Nanomaterials, 15(2), 126. https://doi.org/10.3390/nano15020126
Abd El Hady, W. E., Abdelmageed, M. E., & El-Emam, G. A. (2024). Diosmin-loaded lipid chitosan hybrid nanoparticles boost the anti-inflammatory, antioxidant, and protective effects against acetic acid-induced colitis in rodents. Journal of Drug Delivery Science and Technology, 98, 105846. https://doi.org/10.1016/j.jddst.2024.105846
Bell, L., Chadwick, M., Puranik, M., Tudor, R., Methven, L., Kennedy, S., & Wagstaff, C. (2020). The Eruca sativa genome and transcriptome: A targeted analysis of sulfur metabolism and glucosinolate biosynthesis pre- and postharvest. Frontiers in Plant Science, 11, 525102. https://doi.org/10.3389/fpls.2020.525102
Bhardwaj, N., Kumar, A., Kaur, P., Arora, S., Bedi, N., Bhatia, A., & others. (2025). Erucin-loaded chitosan coated solid lipid hybrid nanoparticles: An efficient drug delivery system for enhancing solubility and sustained release. International Journal of Biological Macromolecules, 308, 142350. https://doi.org/10.1016/j.ijbiomac.2025.142350
Cheng, X., Xie, Q., & Sun, Y. (2023). Advances in nanomaterial-based targeted drug delivery systems. Frontiers in Bioengineering and Biotechnology, 11, 1177151. https://doi.org/10.3389/fbioe.2023.1177151
Cheng, X., Zou, Q., Zhang, H., Zhu, J., Hasan, M., Dong, F., Liu, X., Li, J., Wu, Y., Lv, X., & Wang, K. (2023). Effects of chitosan nanoparticle encapsulation on the properties of litchi polyphenols. Food Science and Biotechnology, 32(13), 1861–1871. https://doi.org/10.1007/s10068-023-013XX
Csóka, I., Ismail, R., Jójárt-Laczkovich, O., & Pallagi, E. (2021). Regulatory considerations, challenges and risk-based approach in nanomedicine development. Current Medicinal Chemistry, 28(36), 7461–7476. https://doi.org/10.2174/0929867328666211103123456
Çiftçi, F., et al. (2025). Advances in drug targeting, drug delivery, and nanotechnology applications: Therapeutic significance in cancer treatment. Pharmaceutics, 17(1), 121. https://doi.org/10.3390/pharmaceutics17010121
Esquivel, R., Juárez, J., Almada, M., Ibarra, J., & Valdez, M. A. (2015). Synthesis and characterization of new thiolated chitosan nanoparticles obtained by ionic gelation method. International Journal of Polymer Science, 2015, 502058. https://doi.org/10.1155/2015/502058
Gomes, A. S., et al. (2024). Chitosan nanoparticles as a potential drug delivery system in the skin: A systematic review based on in vivo studies. ChemistrySelect, 9(202402058). https://doi.org/10.1002/slct.202402058
Gonçalves, M. M., Maluf, D. F., Pontarolo, R., Saul, C. K., Almouazen, E., & Chevalier, Y. (2023). Negatively charged chitosan nanoparticles prepared by ionotropic gelation for encapsulation of positively charged proteins. International Journal of Pharmaceutics, 642, 123164. https://doi.org/10.1016/j.ijpharm.2023.123164
Gutiérrez-Ruíz, S. C., Cortes, H., González-Torres, M., Almarhoon, Z. M., Gürer, E. S., Sharifi-Rad, J., & Leyva-Gómez, G. (2024). Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes. Journal of Biological Engineering, 18(1), 12. https://doi.org/10.1186/s13036-024-00312-3
Habtemariam, S. (2024). Anti-inflammatory therapeutic mechanisms of isothiocyanates: Insights from sulforaphane. Biomedicines, 12(6), 1169. https://doi.org/10.3390/biomedicines12061169
Hanschen, F. S., Lamy, E., Schreiner, M., & Rohn, S. (2014). Reactivity and stability of glucosinolates and their breakdown products in foods. Angewandte Chemie International Edition, 53(43), 11430–11450. https://doi.org/10.1002/anie.201402189
Herdiana, Y., et al. (2024). Drug loading in chitosan-based nanoparticles. Pharmaceutics, 16(8), 1043. https://doi.org/10.3390/pharmaceutics16081043
Hoch, C. C., et al. (2024). Isothiocyanates in medicine: A comprehensive review on phenylethyl-, allyl-, and benzyl-isothiocyanates. Pharmacological Research, 107107. https://doi.org/10.1016/j.phrs.2024.107107
Jha, R., et al. (2024). On the structural and molecular properties of PEO and PEO-PPG functionalized chitosan nanoparticles for drug delivery. Bioengineering, 11(4), 372. https://doi.org/10.3390/bioengineering11040372
Jiang, T., Wang, Y., Yu, Z., & Du, L. (2024). Synthesis and characterization of chitosan/tripolyphosphate nanoparticles loaded with herbicidal compounds. Scientific Reports, 14, 18754. https://doi.org/10.1038/s41598-024-18754-0
Kim, M., et al. (2024). Transformative impact of nanocarrier-mediated drug delivery: Overcoming biological barriers and expanding therapeutic horizons. Small Science. https://doi.org/10.1002/smsc.202400280
Kyriakou, S., et al. (2024). Naturally derived phenethyl isothiocyanate modulates induction of oxidative stress in malignant melanoma. Antioxidants, 13(1), 82. https://doi.org/10.3390/antiox13010082
Leonard, D. N., Chandler, G. W., & Seraphin, S. (2012). Scanning electron microscopy. In Characterization of materials (pp. 1–16). https://doi.org/10.1002/0471266965.COM081.PUB2
Mardani, A., Streimikiene, D., Zavadskas, E. K., Cavallaro, F., Nilashi, M., Jusoh, A., & Zare, H. (2017). Application of structural equation modeling (SEM) to solve environmental sustainability problems: A comprehensive review and meta-analysis. Sustainability, 9(10), 1814. https://doi.org/10.3390/su9101814
Mercan, G., & Selçuk, Z. V. (2024). Progress in utilizing chitosan-based nanoparticles for pulmonary drug administration. ODÜ Tıp Dergisi. https://doi.org/10.56941/odutip.1442818
Moldoveanu, S. C., & David, V. (2021). Modern sample preparation for chromatography. Elsevier.
Narra, F., Galgani, G., Harris, C. B., Moreno, D. A., & Núñez-Gómez, V. (2025). Bioavailability, human metabolism, and dietary interventions of glucosinolates and isothiocyanates: Critical insights and future perspectives. Foods, 14(16), 2876. https://doi.org/10.3390/foods14162876
Omidian, H., et al. (2024). Chitosan nanoparticles for intranasal drug delivery. Pharmaceutics, 16(6), 746. https://doi.org/10.3390/pharmaceutics16060746
Poulev, A., O’Neal, J. M., Logendra, S., et al. (2003). Elicitation, a new window into plant chemodiversity and phytochemical drug discovery. Journal of Medicinal Chemistry, 46(12), 2542–2547. https://doi.org/10.1021/jm030006g
Romeo, L., Iori, R., Rollin, P., Bramanti, P., & Mazzon, E. (2018). Isothiocyanates: An overview of their antimicrobial activity against human infections. Molecules, 23(3), 624. https://doi.org/10.3390/molecules23030624
Shi, S., et al. (2024). Research and application of chitosan nanoparticles in orthopedic infections. International Journal of Nanomedicine, 19, 468848. https://doi.org/10.2147/IJN.S468848
Sikorski, D., Gzyra-Jagieła, K., & Draczyński, Z. (2021). The kinetics of chitosan degradation in organic acid solutions. Marine Drugs, 19(5), 236. https://doi.org/10.3390/md19050236
Singh, S., Singh, G., Attri, S., et al. (2023). Development and optimization of nanoparticles loaded with erucin. Frontiers in Pharmacology, 13, 1080977. https://doi.org/10.3389/fphar.2023.1080977
Sreekumar, S., Goycoolea, F. M., Moerschbacher, B. M., & Rivera-Rodriguez, G. R. (2018). Parameters influencing the size of chitosan-TPP nano- and microparticles. Scientific Reports, 8, 4695. https://doi.org/10.1038/s41598-018-23043-0
Tang, L., & Zhang, Y. (2005). Mitochondria are the primary target in isothiocyanate-induced apoptosis in human bladder cancer cells. Molecular Cancer Therapeutics, 4(8), 1250–1259.
Wang, Q., et al. (2024). Dietary isothiocyanates and anticancer agents: Exploring synergism for improved cancer management. Frontiers in Nutrition, 11, 1386083. https://doi.org/10.3389/fnut.2024.1386083
Yadav, S., et al. (2024). Chitosan-based nanoformulations: Preclinical investigations, theranostic advancements, and clinical trial prospects for targeting diverse pathologies. AAPS PharmSciTech, 25, 248. https://doi.org/10.1208/s12249-024-02948-x
Zhang, Y., et al. (2023). Iberverin exhibits antineoplastic activities against hepatocellular carcinoma. Frontiers in Pharmacology, 14, 1326346. https://doi.org/10.3389/fphar.2023.1326346
Zubair, M., et al. (2024). Application of nanotechnology for targeted drug delivery and nontoxicity. International Journal of Green Pharmacy and Nanomedicine, 2(2), 7436.
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