Fabrication and characterization of Chitosan Nanocarriers for Erucin Delivery Using Ionic Gelation Techniques
DOI:
https://doi.org/10.17977/um067v6i62026p3Keywords:
Chitosan Nanoparticles, Erucin, Ionic Gelation, Eruca Sativa , Drug Delivery SystemAbstract
Background: The incorporation of nanotechnology and bioactive molecules represents a boundary in modern pharmacology, aiming to improve the bioavailability and stability of plant-derived chemicals. This study focuses on the evolution of a novel drug delivery system using chitosan nanocarriers to encapsulate erucin, a potent isothiocyanate derived from Eruca sativa known for its significant anticancer, anti-inflammatory, and antimicrobial properties.
Methods: Fresh chopped Eruca sativa leaves were macerated in water to get erucin phytochemical by enzymatic conversion with myrosinase , followed by an organic solvent extraction using dichloromethane. In the production of nano erucin, sodium tripolyphosphate -a cross-linker - was used in a process called ionic gelation. Three definite solutions were prepared with chitosan-to-erucin ratios of 0.5:1, 1:1, and 1.5:1. Then, centrifugation performed to purify the resulting nanoparticles which were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), and Zeta potential analysis to evaluate morphology, elemental composition, and surface charge, respectively
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